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Issue 6, 2013
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Suspended microtubules demonstrate high sensitivity and low experimental variability in kinesin bead assay

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Abstract

Microtubule (MT) based intraneuronal transport deficiency is directly linked to neurodegeneration. Hence, the development of a reliable and sensitive in vitro approach permitting efficient analysis of MT-based transport is essential for our understanding of the underlying molecular mechanisms that may lead to novel therapeutic approaches for treating neurodegenerative diseases. Here, based on previously developed reconstructed MT–kinesin assay, we propose its “suspended” modification that shows higher sensitivity and lower experimental variability.

Graphical abstract: Suspended microtubules demonstrate high sensitivity and low experimental variability in kinesin bead assay

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Article information


Submitted
08 Aug 2012
Accepted
24 Jan 2013
First published
24 Jan 2013

Analyst, 2013,138, 1653-1656
Article type
Communication

Suspended microtubules demonstrate high sensitivity and low experimental variability in kinesin bead assay

M. C. Tarhan, Y. Orazov, R. Yokokawa, S. L. Karsten and H. Fujita, Analyst, 2013, 138, 1653
DOI: 10.1039/C3AN36545J

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