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Issue 23, 2013
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A visible light photoelectrochemical sensor for tumor marker detection using tin dioxide quantum dot–graphene as labels

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Abstract

In this paper, a simple and sensitive sandwich-type photoelectrochemical (PEC) immunosensor for measurement of biomarkers on a gold nanoparticle-modified indium tin oxide (ITO) electrode through electrodeposition for point-of-care testing was developed by using a tin dioxide quantum dot–graphene nanocomposite (G–SnO2) as an excellent label with amplification techniques. The capture antibody (Ab1) was firstly immobilized on the gold nanoparticle-modified ITO electrode due to the covalent conjugation, then the antigen and the AuNP/PDDA–G–SnO2 nanocomposite nanoparticle labeled signal antibody (Ab2) were conjugated successively to form a sandwich-type immunocomplex through a specific interaction. Under irradiation with a common ultraviolet lamp (∼365 nm, price $50), the SnO2 NPs were excited and underwent charge-separation to yield electrons (e) and holes (h+). As the holes were scavenged by ascorbic acid (AA), the electrons were transferred to the ITO electrode through RGO to generate a photocurrent. The photocurrents were proportional to the CEA concentrations, and the linear range of the developed immunosensor was from 0.005 to 10 ng mL−1 with a detection limit of 0.036 pg mL−1. The proposed sensor shows high sensitivity, stability, reproducibility, and can become a promising platform for other biomolecular detection.

Graphical abstract: A visible light photoelectrochemical sensor for tumor marker detection using tin dioxide quantum dot–graphene as labels

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Article information


Submitted
24 Jul 2013
Accepted
25 Sep 2013
First published
26 Sep 2013

Analyst, 2013,138, 7112-7118
Article type
Paper

A visible light photoelectrochemical sensor for tumor marker detection using tin dioxide quantum dot–graphene as labels

Y. Wang, M. Li, Y. Zhu, S. Ge, J. Yu, M. Yan and X. Song, Analyst, 2013, 138, 7112
DOI: 10.1039/C3AN01410J

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