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Issue 21, 2013
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Analysis of intracellular enzyme activity by surface enhanced Raman scattering

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Abstract

Dysfunctional intracellular enzymatic activity is believed to be an underlying cause of a myriad of diseases. We present the first use of surface enhanced Raman scattering (SERS) as a detection technique capable of reporting intracellular activity of a specific enzyme. Careful choice of reagents allowed the preparation of high resolution cellular activity maps highlighting the specific conversion of the commonly used ELISA reagent 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), by wild type β-galactosidase enzymes. Further, through co-addition of X-Gal substrate and inhibitors we were able to demonstrate that intracellular substrate conversion occurred predominantly through an enzymatically specific pathway. The data presented therefore supports the application of SERS probes as sensitive, specific sensors of biochemical activity and demonstrates the use of SERS probes for the first time as beacons capable of high resolution subcellular localisation of native enzymes.

Graphical abstract: Analysis of intracellular enzyme activity by surface enhanced Raman scattering

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Supplementary files

Article information


Submitted
11 Apr 2013
Accepted
28 Aug 2013
First published
29 Aug 2013

This article is Open Access

Analyst, 2013,138, 6331-6336
Article type
Paper

Analysis of intracellular enzyme activity by surface enhanced Raman scattering

R. Stevenson, S. McAughtrie, L. Senior, R. J. Stokes, H. McGachy, L. Tetley, P. Nativo, J. M. Brewer, J. Alexander, K. Faulds and D. Graham, Analyst, 2013, 138, 6331
DOI: 10.1039/C3AN00729D

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