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Issue 19, 2013
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Dual detection of cancer biomarker CA125 using absorbance and electrochemical methods

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Abstract

An enzyme-linked immunoassay based on dual signal transduction mechanisms has been developed for detection of ovarian cancer biomarker CA125. The immunoassay uses a nanoelectrode array (NEA) chip and absorbance methods for the dual detection. The NEA is used to confirm the optical detection of CA125 that is carried out in a high-binding 96-well plate. An alkaline phosphatase (AP) enzyme was used to label the detection antibody to allow for both the optical and electrochemical detection of CA125. Two kinds of substrates were catalyzed by the AP enzyme. para-Nitrophenylphosphate (PNPP) produces chromogenic para-nitrophenol (PNP), which can be optically detected at 405 nm. para-Aminophenylphosphate (PAPP) produces electroactive para-aminophenol (PAP), which can be detected amperometrically between −0.1 and 0.3 V. The linear ranges have been determined to be 5–1000 U mL−1 and 5–1000 U mL−1 for the optical and electrochemical immunoassays, respectively. The limit of detection of the optical immunoassay is 1.3 U mL−1 and 40 U mL−1 for the optical and electrochemical methods, respectively.

Graphical abstract: Dual detection of cancer biomarker CA125 using absorbance and electrochemical methods

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Publication details

The article was received on 04 Apr 2013, accepted on 16 Jul 2013 and first published on 17 Jul 2013


Article type: Paper
DOI: 10.1039/C3AN00668A
Analyst, 2013,138, 5647-5653

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    Dual detection of cancer biomarker CA125 using absorbance and electrochemical methods

    I. Al-Ogaidi, Z. P. Aguilar, S. Suri, H. Gou and N. Wu, Analyst, 2013, 138, 5647
    DOI: 10.1039/C3AN00668A

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