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Issue 11, 2013
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Tagging the rolling circle products with nanocrystal clusters for cascade signal increase in the detection of miRNA

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Abstract

A new method to label and detect the long ssDNA products from rolling circle (RC) amplification was reported. ZnS nanocrystal clusters (NCCs) were used to tag the RC products. The NCCs release millions of cations to trigger millions of metal-responsive dyes for cascade signal amplification. Selective and sensitive quantification of miRNA was demonstrated; and the NCCs delivered much lower detection limits compared to using peroxidase or quantum dots as the labels.

Graphical abstract: Tagging the rolling circle products with nanocrystal clusters for cascade signal increase in the detection of miRNA

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Article information


Submitted
26 Feb 2013
Accepted
28 Mar 2013
First published
28 Mar 2013

Analyst, 2013,138, 3121-3125
Article type
Communication

Tagging the rolling circle products with nanocrystal clusters for cascade signal increase in the detection of miRNA

J. Yao, K. Flack, L. Ding and W. Zhong, Analyst, 2013, 138, 3121
DOI: 10.1039/C3AN00398A

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