Jump to main content
Jump to site search

Issue 2, 2013
Previous Article Next Article

Real-time isothermal RNA amplification of toxic marine microalgae using preserved reagents on an integrated microfluidic platform

Author affiliations

Abstract

Quantitation of specific RNA sequences is a useful technique in marine biology that can elucidate cell abundance, speciation and viability, especially for early detection of harmful algal blooms. We are thus developing an integrated microfluidic system for cell concentration and lysis, RNA extraction/purification and quantitative RNA detection for environmental applications. The portable system is based on a microfluidic cartridge, or “lab-card”, using a low-cost injection moulded device, with a laminated lid. Here we present real-time isothermal RNA amplification using reagent master-mixes preserved on-chip in a gel at 4 °C for up to eight months. We demonstrate quantitation by reference to an internal control in a competitive assay with 500 cell equivalents of the toxic microalga Karenia brevis. Annealing of primers, amplification at 41 °C and real-time fluorescence detection of the internal control and target using sequence-specific molecular beacons were all performed on-chip.

Graphical abstract: Real-time isothermal RNA amplification of toxic marine microalgae using preserved reagents on an integrated microfluidic platform

Back to tab navigation

Supplementary files

Publication details

The article was received on 17 Jun 2012, accepted on 02 Nov 2012 and first published on 05 Nov 2012


Article type: Paper
DOI: 10.1039/C2AN36464F
Analyst, 2013,138, 593-602

  •   Request permissions

    Real-time isothermal RNA amplification of toxic marine microalgae using preserved reagents on an integrated microfluidic platform

    M. Tsaloglou, F. Laouenan, C. Loukas, L. G. Monsalve, C. Thanner, H. Morgan, J. M. Ruano-López and M. C. Mowlem, Analyst, 2013, 138, 593
    DOI: 10.1039/C2AN36464F

Search articles by author

Spotlight

Advertisements