Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 3, 2012
Previous Article Next Article

In situ formation of silvernanostructures within a polysaccharide film and application as a potential biocompatible fluorescence sensing medium

Author affiliations

Abstract

Simple to manufacture polysaccharide films containing a silver salt, from which silver nanostructures can be produced in situ by light irradiation, were investigated for possible biosensing applications. The silver nanostructures were patterned in situ within a film and cast from a liquid solution of gellan gum, using a compact time-resolved fluorescence microscope. The position and time of irradiation, made using a semiconductor laser in CW mode, were computer controlled. Evidence for their formation was obtained via UV-vis spectroscopy, AFM and SEM-EDAX. On drying the polysaccharide film exhibited a viscosity increase of several orders of magnitude, which was elucidated by changes in the fluorescence lifetime of a probe molecule (DASPMI). To demonstrate the potential for biocompatible sensing applications the influence of the presence of areas of silver nanostructures on the fluorescence of a protein (bovine serum albumin) labelled with fluorescein isothiocyanate was monitored via fluorescence lifetime imaging and the photophysical behaviour found to be consistent with a metal induced increase in the radiative decay rate.

Graphical abstract: In situ formation of silver nanostructures within a polysaccharide film and application as a potential biocompatible fluorescence sensing medium

Back to tab navigation

Supplementary files

Article information


Submitted
22 Sep 2011
Accepted
08 Nov 2011
First published
01 Dec 2011

Soft Matter, 2012,8, 653-659
Article type
Paper

In situ formation of silver nanostructures within a polysaccharide film and application as a potential biocompatible fluorescence sensing medium

G. Hungerford, M. Toury, D. McLoskey, N. Donaldson and A. S. Holmes-Smith, Soft Matter, 2012, 8, 653
DOI: 10.1039/C1SM06813J

Social activity

Search articles by author

Spotlight

Advertisements