Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 5, 2012
Previous Article Next Article

Contact-controlled amoeboid motility induces dynamic cell trapping in 3D-microstructured surfaces

Author affiliations

Abstract

On flat substrates, several cell types exhibit amoeboid migration, which is characterized by restless stochastic successions of pseudopod protrusions. The orientation and frequency of new membrane protrusions characterize efficient search modes, which can respond to external chemical stimuli as observed during chemotaxis in amoebae. To quantify the influence of mechanical stimuli induced by surface topography on the migration modes of the amoeboid model organism Dictyostelium discoideum, we apply high resolution motion analysis in microfabricated pillar arrays of defined density and geometry. Cell motion is analyzed by a two-state motility-model, distinguishing directed cellular runs from phases of isotropic migration that are characterized by randomly oriented cellular protrusions. Cells lacking myosin II or cells deprived of microtubules show significantly different behavior concerning migration velocities and migrational angle distribution, without pronounced attraction to pillars. We conclude that microtubules enhance cellular ability to react with external 3D structures. Our experiments on wild-type cells show that the switching from randomly formed pseudopods to a stabilized leading pseudopod is triggered by contact with surface structures. These alternating processes guide cells according to the available surface in their 3D environment, which we observed dynamically and in steady-state situations. As a consequence, cells perform “home-runs” in low-density pillar arrays, crawling from pillar to pillar, with a characteristic dwell time of ∼75 s. At the boundary between a flat surface and a 3D structured substrate, cells preferentially localize in contact with micropillars, due to the additionally available surface in the microstructured arrays. Such responses of cell motility to microstructures might open new possibilities for cell sorting in surface structured arrays.

Graphical abstract: Contact-controlled amoeboid motility induces dynamic cell trapping in 3D-microstructured surfaces

Back to tab navigation

Supplementary files

Article information


Submitted
07 Apr 2011
Accepted
07 Nov 2011
First published
09 Dec 2011

Soft Matter, 2012,8, 1473-1481
Article type
Paper

Contact-controlled amoeboid motility induces dynamic cell trapping in 3D-microstructured surfaces

D. Arcizet, S. Capito, M. Gorelashvili, C. Leonhardt, M. Vollmer, S. Youssef, S. Rappl and D. Heinrich, Soft Matter, 2012, 8, 1473
DOI: 10.1039/C1SM05615H

Social activity

Search articles by author

Spotlight

Advertisements