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Issue 30, 2012
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Bioinspired and sustainable chitosan-based monoliths for antibody capture and release

Telma Barroso,a   Ana C. A. Roque*a  and  Ana Aguiar-Ricardo*a  
Author affiliations

* Corresponding authors

a REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal
E-mail: cecilia.roque@fct.unl.pt, air@fct.unl.pt

Abstract

Chitosan-based monoliths activated by plasma technology induced the coupling of a robust biomimetic ligand, previously reported as an artificial Protein A, with high yields while minimizing the environmental impact of the procedure. Due to the high porosity, good mechanical and tunable physicochemical properties of the affinity chitosan-based monoliths, it is possible to achieve high binding capacities (150 ± 10 mg antibody per gram support), and to recover 90 ± 5% of the bound protein with 98% purity directly from cell-culture extracts. Therefore, the chitosan-based monoliths prepared by clean processes exhibit a remarkable performance for the one-step capture and recovery of pure antibodies or other biological molecules with biopharmaceutical relevance.

Graphical abstract: Bioinspired and sustainable chitosan-based monoliths for antibody capture and release

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Article information

Submitted
03 Aug 2012
Accepted
18 Sep 2012
First published
18 Sep 2012
RSC Adv., 2012,2, 11285-11294
Article type
Paper
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Bioinspired and sustainable chitosan-based monoliths for antibody capture and release

T. Barroso, A. C. A. Roque and A. Aguiar-Ricardo, RSC Adv., 2012, 2, 11285
DOI: 10.1039/C2RA21687F

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