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Issue 18, 2012
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Direct production of glucose from glycogen under microwave irradiation

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Abstract

The production of fermentable sugars from renewable sources is a challenge. An attempt was made to exploit glycogen as a potential feedstock for the production of glucose. The microwave-assisted acidic hydrolysis was applied for glycogen decomposition for the first time. The optimal conditions for the hydrolysis reaction (yield of glucose – 62 wt.%) were identified: microwave irradiation time – 10 min and concentration of acid – 1 M HCl. Microwave irradiation has dramatically reduced the reaction time from more than 6 h (at 80 °C under an oil bath) to 10 min. 13C NMR spectroscopy was employed to monitor the progress of the hydrolysis reaction. HPLC analysis was employed to evaluate the yield of glucose. Thus, the viability of the use of glycogen as an economically and environmentally benign precursor to the production of glucose has been demonstrated.

Graphical abstract: Direct production of glucose from glycogen under microwave irradiation

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Supplementary files

Article information


Submitted
29 May 2012
Accepted
31 May 2012
First published
13 Jun 2012

RSC Adv., 2012,2, 7262-7267
Article type
Paper

Direct production of glucose from glycogen under microwave irradiation

M. Klein, I. N. Pulidindi, N. Perkas, E. Meltzer-Mats, A. Gruzman and A. Gedanken, RSC Adv., 2012, 2, 7262
DOI: 10.1039/C2RA21066E

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