Issue 20, 2012
From the journal:

RSC Advances

Accurate and effective live bacteria microarray patterning on thick polycationic polymer layers co-patterned with HMDS†

Ieong Wong,‡a   Xianting Ding,‡a   Chunsheng Wub  and  Chih-Ming Ho*a  

* Corresponding authors

a Department of Mechanical and Aerospace Engineering, School of Engineering and Applied Science, University of California, Los Angeles 420 Westwood PlazaLos Angeles, CA, USA
E-mail: chihming@ucla.edu
Fax: +1 (310) 206 2302
Tel: +1 (310) 825 9993

b Biosensor National Special Laboratory, MOE Key Laboratory of Biomedical Engineering, Department of Biomedical Engineering, Zhejiang University, Hangzhou, P. R. China

Abstract

A new bacteria microarray patterning technique is developed by patterning thick polycationic polymers on a glass surface, which generates high-coverage and high-precision E. coli cell patterns. Cell immobilization efficiency is greatly improved, compared to that of the conventional monolayer surface patterning approach. Cell viability tests show very low cytotoxicity of polyethyleneimine (PEI). This advancement should further accelerate biomedical and bacteriological research on the micro scale.

Graphical abstract: Accurate and effective live bacteria microarray patterning on thick polycationic polymer layers co-patterned with HMDS

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Article information

DOI
https://doi.org/10.1039/C2RA20938A
Article type
Communication
Submitted
15 May 2012
Accepted
01 Jul 2012
First published
03 Jul 2012

RSC Adv., 2012,2, 7673-7676

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Accurate and effective live bacteria microarray patterning on thick polycationic polymer layers co-patterned with HMDS

I. Wong, X. Ding, C. Wu and C. Ho, RSC Adv., 2012, 2, 7673 DOI: 10.1039/C2RA20938A

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