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Issue 20, 2012
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A novel fluorescent probe with high sensitivity and selective detection of lipid hydroperoxides in cells

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Abstract

Spy-LHP was developed as a fluorescent probe for the detection and imaging of lipid hydroperoxides in living cells. Although Spy-LHP detection of lipid hydroperoxides is sensitive and selective, this probe is unsuitable for live-cell imaging because of its high hydrophobicity. To overcome this limitation, 2-(4-diphenylphosphanyl-phenyl)-9-(3,6,9,12-tetraoxatridecyl)-anthra[2,1,9-def:6,5,10-def′]diisoquinoline-1,3,8,10-tetraone, Liperfluo, was developed. Liperfluo is structurally similar to Spy-LHP, but is much more soluble in various organic solvents, such as ethanol and dimethylsulfoxide. The probe was successfully used to image lipid hydroperoxides in SH-SY5Y cells in which lipid peroxidation had been stimulated with 2,2′-azobis[2-(2-imidazolin-2-yl)propane] dihydrochloride or cumene hydroperoxide by using fluorescent microscopy and confocal laser scanning microscopy. Additionally, flow cytometric analysis showed that Liperfluo was four times more sensitive in detecting lipid hydroperoxides than Spy-LHP. These results suggest that Liperfluo is a useful fluorescent probe for investigating the roles of lipid peroxidation in a variety of cell pathophysiologies.

Graphical abstract: A novel fluorescent probe with high sensitivity and selective detection of lipid hydroperoxides in cells

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Article information


Submitted
30 Apr 2012
Accepted
25 Jun 2012
First published
01 Aug 2012

RSC Adv., 2012,2, 7894-7900
Article type
Paper

A novel fluorescent probe with high sensitivity and selective detection of lipid hydroperoxides in cells

K. Yamanaka, Y. Saito, J. Sakiyama, Y. Ohuchi, F. Oseto and N. Noguchi, RSC Adv., 2012, 2, 7894
DOI: 10.1039/C2RA20816D

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