A microfluidic platform for osmotic fragility test of red blood cells

Lei Li; Jing Su; Jing Li; Fei Peng; Hongkai Wu; Datian Ye; Hongda Chen

doi:10.1039/C2RA20051A

A microfluidic platform for osmotic fragility test of red blood cells

Lei Li,^a^b Jing Su,^c Jing Li,^d Fei Peng,^e^f Hongkai Wu,*^b^c Datian Ye*^e^f and Hongda Chen^a

* Corresponding authors

^a State Key Laboratory of Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China

^b WPI Advanced Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Japan
E-mail: chhuwu@ust.hk

^c Department of Chemistry, The Hong Kong University of Science & Technology, Hong Kong, China

^d Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing, China

^e Department of Biomedical Engineering, Medical School, Tsinghua University, Beijing, China
E-mail: yedt6386@sz.tsinghua.edu.cn

^f Research Center of Biomedical Engineering, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China

Abstract

An osmotic fragility test is a useful way to determine the extent of red blood cell (RBC) hemolysis resulting from osmotic stress. A microfluidic chip-based system for measuring the osmotic fragility of RBCs has been developed. The chip was made from a Y-shaped polydimethylsiloxane (PDMS) microchannel sealed to a glass cover plate. Fresh rabbit blood diluted 1 : 10 with an isotonic solution and pure water respectively were introduced into the long serpentine channel using two syringe pumps. Hypotonic saline solutions with three different NaCl concentrations were prepared on a chip and images of RBCs at different locations in the channel were captured. The extent of hemolysis was estimated by comparing the cell numbers in the images using an automatic image processing program. Different degrees of hemolysis (no hemolysis, partial hemolysis, and complete hemolysis) can be estimated with this platform. This device provides a promising screening platform for diseases marked by RBC abnormalities with great simplicity, high speed and minimal requirement of blood samples.

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Publication details

The article was received on 09 Jan 2012, accepted on 27 May 2012 and first published on 30 May 2012

Article type: Paper

DOI: 10.1039/C2RA20051A

Citation: RSC Adv., 2012,2, 7161-7165

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A microfluidic platform for osmotic fragility test of red blood cells

L. Li, J. Su, J. Li, F. Peng, H. Wu, D. Ye and H. Chen, RSC Adv., 2012, 2, 7161
DOI: 10.1039/C2RA20051A
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A microfluidic platform for osmotic fragility test of red blood cells

Abstract

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A microfluidic platform for osmotic fragility test of red blood cells

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