Jump to main content
Jump to site search

Issue 6, 2012
Previous Article Next Article

Investigation of glycopolymer–lectin interactions using QCM-d: comparison of surface binding with inhibitory activity

Author affiliations

Abstract

Glycopolymers offer many opportunities for interfacing synthetic materials with biological systems. However, the nature of the interactions between glycopolymers and their biological targets, lectins, and the structural features necessary to obtain high-affinity materials are not fully understood. Here, the enhancement in binding affinity of multivalent glycopolymers to their corresponding lectins is investigated by quartz-crystal microbalance with dissipation monitoring (QCM-d). This technique allows the conformation of the adsorbed polymers to be probed and the direct observation of spanning of multiple binding sites on lectin-functional surfaces. The measured affinity was compared to the anti-adhesion activity of the polymers in solution, and it is shown that increased association constants did not directly correlate with inhibitory activity.

Graphical abstract: Investigation of glycopolymer–lectin interactions using QCM-d: comparison of surface binding with inhibitory activity

Back to tab navigation

Article information


Submitted
09 Mar 2012
Accepted
29 Mar 2012
First published
27 Apr 2012

Polym. Chem., 2012,3, 1634-1640
Article type
Paper

Investigation of glycopolymer–lectin interactions using QCM-d: comparison of surface binding with inhibitory activity

Y. Gou, S. Richards, D. M. Haddleton and M. I. Gibson, Polym. Chem., 2012, 3, 1634 DOI: 10.1039/C2PY20140B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements