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Issue 11, 2012
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Fluorescence photoswitching and photoreversible two-way energy transfer in a photochrome–fluorophore dyad

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Abstract

A thorough photophysical study of a photochrome–fluorophore dyad (3), combining a fluorescent laser dye (DCM-type, 1, Φ1 = 0.27) and a photochromic diarylethene (2), obtained by click chemistry, is presented. In addition to photochromism, the open form (OF) of 2 exhibits fluorescence (Φ22-OF = 0.016), whereas the closed form (CF) does not. Fluorescence is switched upon alternate UV/visible irradiation of 2. The emission band of 2-OF matches the absorption band of 1 (400–550 nm), whereas the emission band of 1 overlaps the absorption band of 2-CF (550–700 nm). Therefore, a photoreversible two-way excitation energy transfer (EET), controlled by the state of the photochromic moiety, is obtained in the dyad 3. Their efficiencies are quantified as ΦEETOF→F = 85% and as ΦEETF→CF ∼ 100% from the comparison of emission and excitation spectra between 1, 2, and 3. These results are fully compatible with the shortening of fluorescence lifetimes (from τ22-OF = 70 ps and 170 ps essentially to τ3333-OF < 10 ps) and to the values of Förster radii determined for 3 (R0(OF → F) = 29 Å and R0(F → CF) = 71 Å), evidencing a Förster-type resonance energy transfer mechanism (FRET). An important outcome of this two-way FRET is the possibility to quench 49% of the fluorescence in 3 at PSS upon UV irradiation, corresponding to the conversion extent of the photochromic reaction, which is different from 2 (αCF = 91%). This is a clear example of a situation where the presence of FRET between the photochromic unit and the fluorophore affects noticeably the photochromic properties of the dyad molecule 3.

Graphical abstract: Fluorescence photoswitching and photoreversible two-way energy transfer in a photochrome–fluorophore dyad

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Article information


Submitted
30 Apr 2012
Accepted
29 Jun 2012
First published
06 Jul 2012

Photochem. Photobiol. Sci., 2012,11, 1705-1714
Article type
Paper

Fluorescence photoswitching and photoreversible two-way energy transfer in a photochrome–fluorophore dyad

K. Ouhenia-Ouadahi, R. Métivier, S. Maisonneuve, A. Jacquart, J. Xie, A. Léaustic, P. Yu and K. Nakatani, Photochem. Photobiol. Sci., 2012, 11, 1705
DOI: 10.1039/C2PP25129A

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