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Issue 43, 2012
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Synthesis of biotin labelled cap analogue – incorporable into mRNA transcripts and promoting cap-dependent translation

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Abstract

Analogues of the eukaryotic messenger RNA 5′ end (m7G cap) are useful tools for studying mRNA fate and serve as reagents for in vitro preparation of 5′ capped mRNAs. We designed a biotin-labeled dinucleotide cap analogue that can be incorporated into transcripts to produce 5′-capped and biotinylated mRNAs which retain their biological functionality and may be employed for biotin–(strept)avidin technologies.

Graphical abstract: Synthesis of biotin labelled cap analogue – incorporable into mRNA transcripts and promoting cap-dependent translation

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Publication details

The article was received on 01 Jun 2012, accepted on 10 Jul 2012 and first published on 13 Jul 2012


Article type: Communication
DOI: 10.1039/C2OB26060C
Org. Biomol. Chem., 2012,10, 8570-8574

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    Synthesis of biotin labelled cap analogue – incorporable into mRNA transcripts and promoting cap-dependent translation

    J. Jemielity, M. Lukaszewicz, J. Kowalska, J. Czarnecki, J. Zuberek and E. Darzynkiewicz, Org. Biomol. Chem., 2012, 10, 8570
    DOI: 10.1039/C2OB26060C

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