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Unprecedented regioselective acylation of flavonoid aglycones was achieved using Candida antarcticalipase B (CALB). The rapid screening of product formation was performed by the use of the high resolution phenol-type OH 1H NMR spectral region recorded after the addition of picric acid.

Graphical abstract: Unexpected enzyme-catalyzed regioselective acylation of flavonoid aglycones and rapid product screening

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