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Issue 7, 2012
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Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling

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Abstract

The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homoduplex or heteroduplex DNA. This approach allows stabilization of various transient MutS–DNA complexes and will enable their structural and functional analysis.

Graphical abstract: Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling

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Article information


Submitted
12 Mar 2012
Accepted
16 Apr 2012
First published
17 Apr 2012

Mol. BioSyst., 2012,8, 1861-1864
Article type
Communication

Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling

R. J. Heinze, S. Sekerina, I. Winkler, C. Biertümpfel, T. S. Oretskaya, E. Kubareva and P. Friedhoff, Mol. BioSyst., 2012, 8, 1861 DOI: 10.1039/C2MB25086A

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