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Issue 3, 2013
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Electrochemical detection of individual DNA hybridization events

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Abstract

We report on real-time electrochemical detection of individual DNA hybridization events at an electrode surface. The experiment is carried out in a microelectrochemical device configured with a working electrode modified with single-stranded DNA probe molecules. When a complementary DNA strand labelled with a catalyst hybridizes to the probe, an easily detectable electrocatalytic current is observed. In the experiments reported here, the catalyst is a platinum nanoparticle and the current arises from electrocatalytic oxidation of hydrazine. Two types of current transients are observed: short bursts and longer-lived steps. At low concentrations of hydrazine, the average size of the current transients is proportional to the amount of hydrazine present, but at higher concentrations the hydrazine oxidation reaction interferes with hybridization.

Graphical abstract: Electrochemical detection of individual DNA hybridization events

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Publication details

The article was received on 30 Aug 2012, accepted on 08 Nov 2012 and first published on 09 Nov 2012


Article type: Paper
DOI: 10.1039/C2LC40993C
Lab Chip, 2013,13, 349-354

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    Electrochemical detection of individual DNA hybridization events

    T. M. Alligrant, E. G. Nettleton and R. M. Crooks, Lab Chip, 2013, 13, 349
    DOI: 10.1039/C2LC40993C

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