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Issue 3, 2013
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Electrochemical detection of individual DNA hybridization events

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Abstract

We report on real-time electrochemical detection of individual DNA hybridization events at an electrode surface. The experiment is carried out in a microelectrochemical device configured with a working electrode modified with single-stranded DNA probe molecules. When a complementary DNA strand labelled with a catalyst hybridizes to the probe, an easily detectable electrocatalytic current is observed. In the experiments reported here, the catalyst is a platinum nanoparticle and the current arises from electrocatalytic oxidation of hydrazine. Two types of current transients are observed: short bursts and longer-lived steps. At low concentrations of hydrazine, the average size of the current transients is proportional to the amount of hydrazine present, but at higher concentrations the hydrazine oxidation reaction interferes with hybridization.

Graphical abstract: Electrochemical detection of individual DNA hybridization events

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Supplementary files

Article information


Submitted
30 Aug 2012
Accepted
08 Nov 2012
First published
09 Nov 2012

Lab Chip, 2013,13, 349-354
Article type
Paper

Electrochemical detection of individual DNA hybridization events

T. M. Alligrant, E. G. Nettleton and R. M. Crooks, Lab Chip, 2013, 13, 349 DOI: 10.1039/C2LC40993C

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