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Issue 20, 2012
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Engineering of polarized tubular structures in a microfluidic device to study calcium phosphate stone formation

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Abstract

This communication describes the formation of tubular structures with a circular cross-section by growing epithelial cells in a microfluidic (MF) device. Here we show for the first time that it is possible to form a monolayer of polarized cells, embedded within the MF device which can function as an in vivo epithelia. We showed: i) the overexpression of specific protein(s) of interest (i.e., ion channel and transport proteins) is feasible inside tubular structures in MFs; ii) the functional kinetic information of Ca2+ in cells can be measured by microflurometry using cell permeable Ca2+ probe under confocal microscope; and iii) calcium phosphate stones can be produced in real time in MFs with Ca2+ transporting epithelia. These data suggest that tubular structures inside this MF platform can be used as a suitable model to understand the molecular and pharmacological basis of calcium phosphate stone formation in the epithelial or other similar cellular micro environments.

Graphical abstract: Engineering of polarized tubular structures in a microfluidic device to study calcium phosphate stone formation

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Publication details

The article was received on 14 Jul 2012, accepted on 23 Aug 2012 and first published on 24 Aug 2012


Article type: Communication
DOI: 10.1039/C2LC40801E
Citation: Lab Chip, 2012,12, 4037-4040
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    Engineering of polarized tubular structures in a microfluidic device to study calcium phosphate stone formation

    Z. Wei, P. K. Amponsah, M. Al-Shatti, Z. Nie and B. C. Bandyopadhyay, Lab Chip, 2012, 12, 4037
    DOI: 10.1039/C2LC40801E

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