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Issue 20, 2012
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Double spiral microchannel for label-free tumor cell separation and enrichment

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Abstract

This work reports on a passive double spiral microfluidic device allowing rapid and label-free tumor cell separation and enrichment from diluted peripheral whole blood, by exploiting the size-dependent hydrodynamic forces. A numerical model is developed to simulate the Dean flow inside the curved geometry and to track the particle/cell trajectories, which is validated against the experimental observations and serves as a theoretical foundation for optimizing the operating conditions. Results from separating tumor cells (MCF-7 and Hela) spiked into whole blood indicate that 92.28% of blood cells and 96.77% of tumor cells are collected at the inner and the middle outlet, respectively, with 88.5% tumor recovery rate at a throughput of 3.33 × 107 cells min−1. We expect that this label-free microfluidic platform, driven by purely hydrodynamic forces, would have an impact on fundamental and clinical studies of circulating tumor cells.

Graphical abstract: Double spiral microchannel for label-free tumor cell separation and enrichment

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Supplementary files

Article information


Submitted
29 Apr 2012
Accepted
02 Jul 2012
First published
04 Jul 2012

Lab Chip, 2012,12, 3952-3960
Article type
Paper

Double spiral microchannel for label-free tumor cell separation and enrichment

J. Sun, M. Li, C. Liu, Y. Zhang, D. Liu, W. Liu, G. Hu and X. Jiang, Lab Chip, 2012, 12, 3952
DOI: 10.1039/C2LC40679A

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