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Issue 13, 2012
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Capacitance-based assay for real-time monitoring of endocytosis and cell viability

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Abstract

Label-free cell-based assays have emerged as a promising means for high-throughput screening. Most label-free sensors are based on impedance measurements that reflect the passive electrical properties of cells. Here we introduce a capacitance-based assay that measures the dielectric constant (capacitance) of biological cells, and demonstrate the feasibility of analyzing endocytosis and screening chemotherapeutic agents with this assay. Endocytosis induces a change in the zeta potential, leading to a change in the dielectric constant which enables real-time endocytosis monitoring using the capacitance sensor. Additionally, since the dielectric constant is proportional to cell radius and cell volume, cell viability can be estimated from the change in capacitance. Therefore, the capacitance sensor array can also be used for cytotoxicity testing for large-scale chemotherapeutic screening.

Graphical abstract: Capacitance-based assay for real-time monitoring of endocytosis and cell viability

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Publication details

The article was received on 12 Dec 2011, accepted on 16 Mar 2012 and first published on 16 Mar 2012


Article type: Paper
DOI: 10.1039/C2LC21236F
Lab Chip, 2012,12, 2377-2384

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    Capacitance-based assay for real-time monitoring of endocytosis and cell viability

    R. Lee, J. Kim, S. Y. Kim, S. M. Jang, S. Lee, I. Choi, S. W. Park, J. Shin and K. Yoo, Lab Chip, 2012, 12, 2377
    DOI: 10.1039/C2LC21236F

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