Issue 6, 2012
From the journal:

Lab on a Chip

Hydrodynamic shearing of DNA in a polymeric microfluidic device†

Irina V. Nesterova,b   Mateusz L. Hupert,ab   Malgorzata A. Witekab  and  Steven A. Soper*abc  

* Corresponding authors

a Department of Biomedical Engineering and Department of Chemistry, University of North Carolina, Chapel Hill, Chapel Hill, NC, USA
E-mail: ssoper@unc.edu

b Department of Chemistry, Louisiana State University, Baton Rouge, LA, USA

c School of Nano-Biotechnology and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, South Korea

Abstract

With the advent of next-generation sequencing (NGS) systems and the associated high throughput they afford, the input to these machines requires manageable lengths of fragments (∼1000 bp) produced from chromosomal DNAs. Therefore, it is critical to develop devices that can shear DNA in a controlled fashion. We report a polymer-based microfluidic device that establishes an efficient and inexpensive platform with performance comparable to a commercially available bench-top system.

Graphical abstract: Hydrodynamic shearing of DNA in a polymeric microfluidic device

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Article information

DOI
https://doi.org/10.1039/C2LC21122J
Article type
Communication
Submitted
17 Nov 2011
Accepted
26 Jan 2012
First published
27 Jan 2012

Lab Chip, 2012,12, 1044-1047

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Hydrodynamic shearing of DNA in a polymeric microfluidic device

I. V. Nesterova, M. L. Hupert, M. A. Witek and S. A. Soper, Lab Chip, 2012, 12, 1044 DOI: 10.1039/C2LC21122J

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