Jump to main content
Jump to site search

Issue 16, 2012
Previous Article Next Article

Limits of miniaturization: Assessing ITP performance in sub-micron and nanochannels

Author affiliations

Abstract

The feasibility of isotachophoresis in channels of sub micrometer and nanometer dimension is investigated. A sample injection volume of 0.4 pL is focused and separated in a 330 nm deep channel. The sample consists of a biomatrix containing the fluorescently-labeled amino acids glutamate and phenylalanine, 20 attomoles of each. Isotachophoretic focusing is successfully demonstrated in a 50 nm deep channel. Separation of the two amino acids in the 50 nm deep channel however, could not be performed as the maximum applicable voltage was insufficient. This limit is imposed by bubble formation that we contribute to cavitation as a result of the mismatch in electro-osmotic flow, so called electrocavitation. This represents an unexpected limit on the miniaturization of ITP. Nonetheless, we report the smallest isotachophoretic separation and focusing experiment to date, both in terms of controlled sample injection volume and channel height.

Graphical abstract: Limits of miniaturization: Assessing ITP performance in sub-micron and nanochannels

Back to tab navigation

Supplementary files

Publication details

The article was received on 19 Oct 2011, accepted on 30 Mar 2012 and first published on 12 Jun 2012


Article type: Paper
DOI: 10.1039/C2LC21011H
Lab Chip, 2012,12, 2888-2893

  •   Request permissions

    Limits of miniaturization: Assessing ITP performance in sub-micron and nanochannels

    K. G. H. Janssen, J. Li, H. T. Hoang, P. Vulto, R. J. B. H. N. van den Berg, H. S. Overkleeft, J. C.T. Eijkel, N. R. Tas, H. J. van der Linden and T. Hankemeier, Lab Chip, 2012, 12, 2888
    DOI: 10.1039/C2LC21011H

Search articles by author

Spotlight

Advertisements