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Issue 3, 2012
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Label-free real-time imaging in microchip free-flow electrophoresis applying high speed deep UV fluorescence scanning

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Abstract

We report on label-free monitoring of microfluidic free-flow electrophoresis (μFFE) separations in real-time using a custom built high speed deep UV laser scanner. In combination with a novel layout realized in fused silica (FS) FFE chips the setup was successfully applied for continuous separations and detection of unlabeled analytes including native proteins by space-resolved intrinsic deep UV fluorescence scanning.

Graphical abstract: Label-free real-time imaging in microchip free-flow electrophoresis applying high speed deep UV fluorescence scanning

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Article information


Submitted
27 Jun 2011
Accepted
19 Sep 2011
First published
19 Oct 2011

Lab Chip, 2012,12, 458-463
Article type
Paper

Label-free real-time imaging in microchip free-flow electrophoresis applying high speed deep UV fluorescence scanning

S. Köhler, S. Nagl, S. Fritzsche and D. Belder, Lab Chip, 2012, 12, 458
DOI: 10.1039/C1LC20558G

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