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Volume 159, 2012
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Precipitation of ACC in liposomes—a model for biomineralization in confined volumes

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Abstract

Biomineralizing organisms frequently precipitate minerals in small phospholipid bilayer-delineated compartments. We have established an in vitro model system to investigate the effect of confinement in attoliter to femtoliter volumes on the precipitation of calcium carbonate. In particular, we analyze the growth and stabilization of liposome-encapsulated amorphous calcium carbonate (ACC) nanoparticles using a combination of in situ techniques, cryo-transmission electron microscopy (Cryo-TEM), and small angle X-ray scattering (SAXS). Herein, we discuss ACC nanoparticle growth rate as a function of liposome size, carbon dioxide flux across the liposome membrane, pH, and osmotic pressure. Based on these experiments, we argue that the stabilization of ACC nanoparticles in liposomes is a consequence of a low nucleation rate (high activation barrier) of crystalline polymorphs of calcium carbonate.

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Supplementary files

Article information


Submitted
30 Apr 2012
Accepted
06 Jun 2012
First published
07 Jun 2012

Faraday Discuss., 2012,159, 345-356
Article type
Paper

Precipitation of ACC in liposomes—a model for biomineralization in confined volumes

C. C. Tester, C. Wu, S. Weigand and D. Joester, Faraday Discuss., 2012, 159, 345
DOI: 10.1039/C2FD20088K

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