Issue 8, 2012

Porous cross linked enzyme aggregates (p-CLEAs) of Saccharomyces cerevisiae invertase

Abstract

In this work, porous cross-linked enzyme aggregates (p-CLEAs) of Saccharomyces cerevisiae invertase are reported for the first time. Porous CLEAs were prepared by adding starch as a pore-making agent in crude invertase solution and co-precipitating with invertase using ammonium sulfate as a precipitating agent. The resultant co-precipitate was cross-linked for 8 h with 20 mM glutaraldehyde and in the end starch was removed by alpha amylase. The highest catalytic efficiency was obtained at 0.4% (w/v) starch. The scanning electron microscopy analysis of invertase p-CLEAs showed a porous structure. The p-CLEAs were shown to have improved internal mass transfer compared to conventional CLEAs.

Graphical abstract: Porous cross linked enzyme aggregates (p-CLEAs) of Saccharomyces cerevisiae invertase

  • This article is part of the themed collection: Biocatalysis

Article information

Article type
Paper
Submitted
08 May 2012
Accepted
08 Jun 2012
First published
13 Jun 2012

Catal. Sci. Technol., 2012,2, 1575-1579

Porous cross linked enzyme aggregates (p-CLEAs) of Saccharomyces cerevisiae invertase

S. Talekar, V. Shah, S. Patil and M. Nimbalkar, Catal. Sci. Technol., 2012, 2, 1575 DOI: 10.1039/C2CY20304A

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