Issue 82, 2012
From the journal:

Chemical Communications

Suppression of matrix ions by N-phosphorylation labeling using matrix-assisted laser desorption–ionization time-of-flight mass spectrometry†

Xiang Gao,ab   Zhi Tang,a   Minghua Lu,a   Hongxia Liu,*b   Yuyang Jiang,b   Yufen Zhaoc  and  Zongwei Cai*ab  

* Corresponding authors

a Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, China
E-mail: zwcai@hkbu.edu.hk
Fax: +86 852 3411 7348
Tel: +86 852 3411 7070

b The Key Laboratory for Cancer Metabolomics of Shenzhen, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China
E-mail: liuhx@sz.tsinghua.edu.cn

c Department of Chemistry and the Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, P. R. China

Abstract

N-Phosphorylation labeling was utilized to analyze small metabolites using matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF MS). The incorporation of a neutral phosphoryl group with high gas-phase proton affinity not only improved the ionization efficiency of the target molecules, but also greatly reduced the matrix background interference.

Graphical abstract: Suppression of matrix ions by N-phosphorylation labeling using matrix-assisted laser desorption–ionization time-of-flight mass spectrometry

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Article information

DOI
https://doi.org/10.1039/C2CC36091H
Article type
Communication
Submitted
19 Jul 2012
Accepted
29 Aug 2012
First published
29 Aug 2012

Chem. Commun., 2012,48, 10198-10200

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Suppression of matrix ions by N-phosphorylation labeling using matrix-assisted laser desorption–ionization time-of-flight mass spectrometry

X. Gao, Z. Tang, M. Lu, H. Liu, Y. Jiang, Y. Zhao and Z. Cai, Chem. Commun., 2012, 48, 10198 DOI: 10.1039/C2CC36091H

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