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Issue 98, 2012
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eLCR: electrochemical detection of single DNA base changes via Ligase Chain Reaction

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Abstract

Simple, inexpensive and wide-scaled analysis of single DNA base changes (‘point mutations’ which include SNP's, somatic mutations and epigenetic changes) is one of the holy grail's of point-of-care diagnostics. Herein, we present eLCR: a simple methodology that fuses Ligase Chain Reaction (LCR) with electrochemical detection based on DNA-mediated charge transport. LCR generates long and short “knife” motifs representing the presence or absence of single DNA base changes, which are then detected electrochemically by either methylene blue intercalation or horseradish peroxidase labelling.

Graphical abstract: eLCR: electrochemical detection of single DNA base changes via Ligase Chain Reaction

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Supplementary files

Article information


Submitted
13 Aug 2012
Accepted
24 Oct 2012
First published
24 Oct 2012

Chem. Commun., 2012,48, 12014-12016
Article type
Communication

eLCR: electrochemical detection of single DNA base changes via Ligase Chain Reaction

E. J. H. Wee, M. J. A. Shiddiky, M. A. Brown and M. Trau, Chem. Commun., 2012, 48, 12014
DOI: 10.1039/C2CC35841G

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