Issue 98, 2012

eLCR: electrochemical detection of single DNA base changes via Ligase Chain Reaction

Abstract

Simple, inexpensive and wide-scaled analysis of single DNA base changes (‘point mutations’ which include SNP's, somatic mutations and epigenetic changes) is one of the holy grail's of point-of-care diagnostics. Herein, we present eLCR: a simple methodology that fuses Ligase Chain Reaction (LCR) with electrochemical detection based on DNA-mediated charge transport. LCR generates long and short “knife” motifs representing the presence or absence of single DNA base changes, which are then detected electrochemically by either methylene blue intercalation or horseradish peroxidase labelling.

Graphical abstract: eLCR: electrochemical detection of single DNA base changes via Ligase Chain Reaction

Supplementary files

Article information

Article type
Communication
Submitted
13 Aug 2012
Accepted
24 Oct 2012
First published
24 Oct 2012

Chem. Commun., 2012,48, 12014-12016

eLCR: electrochemical detection of single DNA base changes via Ligase Chain Reaction

E. J. H. Wee, M. J. A. Shiddiky, M. A. Brown and M. Trau, Chem. Commun., 2012, 48, 12014 DOI: 10.1039/C2CC35841G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements