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Issue 88, 2012
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Direct immobilization of oxyamine-modified proteins from cell lysates

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Abstract

Oxyamine-modified proteins can be efficiently and selectively immobilized on ketone-coated glass slides at neutral pH in short reaction times by direct treatment and spotting of protein expression lysates without prior purification.

Graphical abstract: Direct immobilization of oxyamine-modified proteins from cell lysates

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Supplementary files

Article information


Submitted
20 Jul 2012
Accepted
11 Sep 2012
First published
11 Sep 2012

Chem. Commun., 2012,48, 10829-10831
Article type
Communication

Direct immobilization of oxyamine-modified proteins from cell lysates

L. Yi, Y. Chen, P. Lin, H. Schröder, Christof. M. Niemeyer, Y. Wu, R. S. Goody, G. Triola and H. Waldmann, Chem. Commun., 2012, 48, 10829
DOI: 10.1039/C2CC35237K

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