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Issue 77, 2012
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Incorporation of 4′-C-aminomethyl-2′-O-methylthymidine into DNA by thermophilic DNA polymerases

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Abstract

The dual modified nucleotide 4′-C-aminomethyl-2′-O-methylthymidine 5′-triphosphate was synthesized and enzymatically incorporated into DNA by the thermophilic DNA polymerases Pfu and Therminator III. The dual ribose modification imparted increased exonuclease resistance to DNA compared to the well-known 2′-O-methyl modification.

Graphical abstract: Incorporation of 4′-C-aminomethyl-2′-O-methylthymidine into DNA by thermophilic DNA polymerases

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Publication details

The article was received on 20 Jul 2012, accepted on 06 Aug 2012 and first published on 07 Aug 2012


Article type: Communication
DOI: 10.1039/C2CC35222B
Chem. Commun., 2012,48, 9619-9621

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    Incorporation of 4′-C-aminomethyl-2′-O-methylthymidine into DNA by thermophilic DNA polymerases

    G. N. Nawale, K. R. Gore, C. Höbartner and P. I. Pradeepkumar, Chem. Commun., 2012, 48, 9619
    DOI: 10.1039/C2CC35222B

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