Jump to main content
Jump to site search

Issue 91, 2012
Previous Article Next Article

Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

Author affiliations

Abstract

Two new dibenzocyclooctyne–thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes.

Graphical abstract: Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

Back to tab navigation

Supplementary files

Additions and corrections

Article information


Submitted
16 Jul 2012
Accepted
01 Aug 2012
First published
03 Aug 2012

Chem. Commun., 2012,48, 11184-11186
Article type
Communication

Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

M. Shelbourne, T. Brown, A. H. El-Sagheer and T. Brown, Chem. Commun., 2012, 48, 11184
DOI: 10.1039/C2CC35084J

Social activity

Search articles by author

Spotlight

Advertisements