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Issue 65, 2012
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Templated chemistry for monitoring damage and repair directly in duplex DNA§

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Abstract

We report the fluorogenic detection of the product of base excision repair (an abasic site) in a specific sequence of duplex DNA. This is achieved by DNA-templated chemistry, employing triple helix-forming probes that contain unnatural nucleobases designed to selectively recognize the site of a missing base. Light-up signals of up to 36-fold were documented, and probes could be used to monitor enzymatic removal of a damaged base.

Graphical abstract: Templated chemistry for monitoring damage and repair directly in duplex DNA

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Article information


Submitted
06 Jun 2012
Accepted
27 Jun 2012
First published
28 Jun 2012

Chem. Commun., 2012,48, 8069-8071
Article type
Communication

Templated chemistry for monitoring damage and repair directly in duplex DNA

S. H. Lee, S. Wang and E. T. Kool, Chem. Commun., 2012, 48, 8069
DOI: 10.1039/C2CC34060G

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