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Issue 89, 2012
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A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge§

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Abstract

A propargyl containing guanosine phosphoramidite was synthesized and incorporated into siRNA, enabling click-ligation with an azido fluorophore onto the nucleobase sugar edge. Duplex stability was not affected by labeling at this new site, which allowed deconvolution of the effects of label, structure and attachment site on RNAi activity.

Graphical abstract: A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge

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Publication details

The article was received on 05 Jun 2012, accepted on 17 Sep 2012 and first published on 18 Sep 2012


Article type: Communication
DOI: 10.1039/C2CC34015A
Chem. Commun., 2012,48, 11014-11016
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    A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge

    S. Seidu-Larry, B. Krieg, M. Hirsch, M. Helm and O. Domingo, Chem. Commun., 2012, 48, 11014
    DOI: 10.1039/C2CC34015A

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