Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases

Amanda Beddows; Nikesh Patel; L. David Finger; John M. Atack; David M. Williams; Jane A. Grasby

doi:10.1039/C2CC33400C

Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases†‡

Amanda Beddows,§^a Nikesh Patel,§^a L. David Finger,^a John M. Atack,¶^a David M. Williams^a and Jane A. Grasby*^a

* Corresponding authors

^a Centre for Chemical Biology, Department of Chemistry, Krebs Institute, University of Sheffield, Sheffield, UK
E-mail: j.a.grasby@sheffield.ac.uk
Tel: +44 (0)1142229478

Abstract

Flap endonucleases (FENs) are proposed to select their target phosphate diester by unpairing the two terminal nucleotides of duplex. Interstrand disulfide crosslinks, introduced by oxidation of thiouracil and thioguanine bases, abolished the specificity of human FEN1 for hydrolysis one nucleotide into the 5′-duplex.

This article is part of the themed collection: Nucleic acids: new life, new materials

Supplementary files

Publication details

The article was received on 10 May 2012, accepted on 10 Jul 2012 and first published on 11 Jul 2012

Article type: Communication

DOI: 10.1039/C2CC33400C

Citation: Chem. Commun., 2012,48, 8895-8897

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Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases

A. Beddows, N. Patel, L. D. Finger, J. M. Atack, D. M. Williams and J. A. Grasby, Chem. Commun., 2012, 48, 8895
DOI: 10.1039/C2CC33400C
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Chemical Communications

Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases†‡

Abstract

Supplementary files

Publication details

Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases

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