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Issue 71, 2012
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Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases

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Abstract

Flap endonucleases (FENs) are proposed to select their target phosphate diester by unpairing the two terminal nucleotides of duplex. Interstrand disulfide crosslinks, introduced by oxidation of thiouracil and thioguanine bases, abolished the specificity of human FEN1 for hydrolysis one nucleotide into the 5′-duplex.

Graphical abstract: Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases

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Publication details

The article was received on 10 May 2012, accepted on 10 Jul 2012 and first published on 11 Jul 2012


Article type: Communication
DOI: 10.1039/C2CC33400C
Chem. Commun., 2012,48, 8895-8897

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    Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases

    A. Beddows, N. Patel, L. D. Finger, J. M. Atack, D. M. Williams and J. A. Grasby, Chem. Commun., 2012, 48, 8895
    DOI: 10.1039/C2CC33400C

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