Issue 50, 2012
From the journal:

Chemical Communications

A sensitive electrochemical biosensor for detection of DNA methyltransferase activity by combining DNA methylation-sensitive cleavage and terminal transferase-mediated extension†

Huiwang Wu,a   Shucheng Liu,a   Jianhui Jiang,*a   Guoli Shena  and  Ruqin Yu*a  

* Corresponding authors

a State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, P. R. China
E-mail: jianhuijiang@hnu.edu.cn, rqyu@hnu.edu.cn
Fax: +86-731-8822782
Tel: +86-731-8822577

Abstract

A novel electrochemical biosensor was developed for activity assay of DNA methyltransferase and its inhibitor based on methylation-sensitive cleavage, which activated a primer for terminal transferase-mediated extension of biotinylated dUTP followed by sensitive detection via enzymatic amplification.

Graphical abstract: A sensitive electrochemical biosensor for detection of DNA methyltransferase activity by combining DNA methylation-sensitive cleavage and terminal transferase-mediated extension

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Article information

DOI
https://doi.org/10.1039/C2CC32397D
Article type
Communication
Submitted
03 Apr 2012
Accepted
01 May 2012
First published
01 May 2012

Chem. Commun., 2012,48, 6280-6282

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A sensitive electrochemical biosensor for detection of DNA methyltransferase activity by combining DNA methylation-sensitive cleavage and terminal transferase-mediated extension

H. Wu, S. Liu, J. Jiang, G. Shen and R. Yu, Chem. Commun., 2012, 48, 6280 DOI: 10.1039/C2CC32397D

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