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Issue 10, 2012
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Cell-selective labeling of bacterial proteomes with an orthogonal phenylalanine amino acid reporter

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Abstract

Orthogonal amino acid reporters allow the selective labeling of different cell types in heterogeneous populations through the expression of engineered aminoacyl tRNA synthetases. Here, we demonstrate that para-ethynylphenylalanine (PEP) can be used as an orthogonal amino acid reporter for efficient selective labeling of an intracellular bacterial pathogen during infection.

Graphical abstract: Cell-selective labeling of bacterial proteomes with an orthogonal phenylalanine amino acid reporter

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Supplementary files

Article information


Submitted
10 Aug 2011
Accepted
02 Nov 2011
First published
11 Nov 2011

Chem. Commun., 2012,48, 1473-1474
Article type
Communication

Cell-selective labeling of bacterial proteomes with an orthogonal phenylalanine amino acid reporter

M. Grammel, P. D. Dossa, E. Taylor-Salmon and H. C. Hang, Chem. Commun., 2012, 48, 1473 DOI: 10.1039/C1CC14939C

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