An optimized isotopic labelling strategy of isoleucine-γ2 methyl groups for solution NMR studies of high molecular weight proteins

Isabel Ayala; Olivier Hamelin; Carlos Amero; Ombeline Pessey; Michael J. Plevin; Pierre Gans; Jérôme Boisbouvier

doi:10.1039/C1CC12932E

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An optimized isotopic labelling strategy of isoleucine-γ₂ methyl groups for solution NMR studies of high molecular weight proteins†‡

Isabel Ayala,§^a Olivier Hamelin,§^b Carlos Amero,^ac Ombeline Pessey,^a Michael J. Plevin,^a Pierre Gans^d and Jérôme Boisbouvier*^a

Author affiliations

* Corresponding authors

^a CNRS, Institut de Biologie Structurale, Grenoble, France
E-mail: jerome.boisbouvier@ibs.fr

^b Université de Grenoble, Laboratoire de Chimie et Biologie des Métaux, 17 avenue des Martyrs, 38054 Grenoble Cedex 9, France

^c Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Mexico

^d CEA, IBS-41 rue J. Horowitz, F-38027 Grenoble Cedex 1, France

Abstract

An efficient synthetic route is proposed to produce 2-hydroxy-2-ethyl-3-oxobutanoate for the specific labelling of Ile methyl-γ₂groups in proteins. The ²H, ¹³C-pattern of the biosynthetic precursor has been designed to optimize magnetization transfer, in large proteins, between these important structural probes and their corresponding backbone nuclei.

This article is part of the themed collection: Emerging Investigators 2012

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Article information

DOI: https://doi.org/10.1039/C1CC12932E
Article type: Communication
Submitted: 18 May 2011
Accepted: 14 Jul 2011
First published: 26 Jul 2011

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Chem. Commun., 2012,48, 1434-1436

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An optimized isotopic labelling strategy of isoleucine-γ₂ methyl groups for solution NMR studies of high molecular weight proteins

I. Ayala, O. Hamelin, C. Amero, O. Pessey, M. J. Plevin, P. Gans and J. Boisbouvier, Chem. Commun., 2012, 48, 1434 DOI: 10.1039/C1CC12932E

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