Simultaneous qualitative and quantitative determination of major polymethoxylated flavonoids in the leaves of Murraya paniculata by RRLC-DAD-ESI-MSn

Abstract

A rapid-resolution liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry (RRLC-DAD-ESI-MSⁿ) method was established to identify and quantify the major bioactive constituents in the leaves of Murraya paniculata (L.) Jack. Seven of the main PMFs were determined by the RRLC method with detection wavelength at 330 nm in 30 minutes. The total PMFs in the samples ranged from 12.688 mg g⁻¹ to 54.997 mg g⁻¹. Meanwhile, sixteen PMFs could be identified from the complex extract of the leaves of M. paniculata by the RRLC-ESI-MS/MS method. The developed RRLC method was validated in terms of good linearity, precision and accuracy. The RRLC-ESI-MS/MS method was simple and useful for rapid identification of principal constituents in M. paniculata.