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Issue 4, 2012
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Direct electron transfer of ferritin on electrodeposited nickel oxide cubic nanoparticles

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Abstract

Immobilization of protein molecules on the surface of nanoparticles has many applications in sensing and biosensing. A variety of covalent and non-covalent methods have been reported for protein immobilization on nanoparticles. In this work, nickel oxide cubic nanoparticles (NiOx NPs) were synthesized by electrodeposition method. Iron storage protein molecules (ferritin) were directly adsorbed on the surface of the nanoparticles by cyclic voltammetry (CV). Electrochemical parameters of immobilized ferritin including formal potential (E0′) and charge transfer coefficient (α) were estimated. The results show that the electrodeposited NPs provide an efficient substrate for the immobilization of ferritin. The prepared biosensor exhibits an electrocatalytic activity for the reduction of hydrogen peroxide.

Graphical abstract: Direct electron transfer of ferritin on electrodeposited nickel oxide cubic nanoparticles

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Article information


Submitted
19 Sep 2011
Accepted
03 Feb 2012
First published
15 Mar 2012

Anal. Methods, 2012,4, 1024-1028
Article type
Paper

Direct electron transfer of ferritin on electrodeposited nickel oxide cubic nanoparticles

A. Mohammadi, A. B. Moghaddam and J. Badraghi, Anal. Methods, 2012, 4, 1024
DOI: 10.1039/C2AY05596A

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