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Issue 3, 2013
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Enzyme-free colorimetric bioassay based on gold nanoparticle-catalyzed dye decolorization

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Abstract

A novel, enzyme-free and aptamer-based colorimetric platform for protein detection has been developed, which takes advantage of aptamer-functionalized magnetic beads (MBs) for target capture, concentration and separation, and aptamer-conjugated gold nanoparticle (AuNP)-catalyzed color bleaching reaction of methyl orange (MO) to generate the colorimetric signals. It was demonstrated that the proposed colorimetric sensing strategy enables simple, cost-effective, sensitive and specific thrombin detection without the use of any enhancing solutions and enzymes. Herein, by naked eye observation, we can detect the human thrombin with a detection limit of approximately 320 pM, which can be further decreased to 30 pM with the help of a UV-vis instrument. In addition, this method also works for targets with two or more binding sites.

Graphical abstract: Enzyme-free colorimetric bioassay based on gold nanoparticle-catalyzed dye decolorization

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Article information


Submitted
24 Sep 2012
Accepted
23 Nov 2012
First published
23 Nov 2012

Analyst, 2013,138, 760-766
Article type
Communication

Enzyme-free colorimetric bioassay based on gold nanoparticle-catalyzed dye decolorization

W. Li, J. Li, W. Qiang, J. Xu and D. Xu, Analyst, 2013, 138, 760
DOI: 10.1039/C2AN36374G

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