Issue 24, 2012
From the journal:

Analyst

Three-dimensional Raman spectroscopic imaging of protein crystals deposited on a nanodroplet

Satoshi Nitahara,a   Masatoshi Maeki,b   Hiroshi Yamaguchi,ac   Kenichi Yamashita,ab   Masaya Miyazaki*ab  and  Hideaki Maedaad  

* Corresponding authors

a Measurement Solution Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 807-1 Shuku, Tosu, Saga 841-0052, Japan
E-mail: m.miyazaki@aist.go.jp
Fax: +81-94281-3627
Tel: +81-942-81-4059

b Department of Molecular and Material Sciences, Interdisciplinary Graduate School of Engineering Sciences, Kyushu University, 6-1 Kasuga-koen, Kasuga, Fukuoka 816-8580, Japan

c Liberal Arts Education Center, Tokai University, Kawayo, Minami-Aso, Kumamoto 869-1404, Japan

d Core Research for Evolutional Science & Technology (CREST), Japan Science and Technology Agency (JST), 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan

Abstract

Confocal Raman spectroscopic imaging has been used to find the location of protein crystals deposited in a nanodroplet. The depth of the protein crystal has been clearly identified by comparing the three-dimensional Raman spectroscopic images of the protein with those of water. Additionally, the low concentration region around a growing protein crystal in the nanodroplet was visualized using two-dimensional Raman spectroscopic imaging.

Graphical abstract: Three-dimensional Raman spectroscopic imaging of protein crystals deposited on a nanodroplet

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/C2AN35942A
Article type
Paper
Submitted
11 Jul 2012
Accepted
02 Oct 2012
First published
02 Oct 2012

Analyst, 2012,137, 5730-5735

Permissions

Request permissions

Three-dimensional Raman spectroscopic imaging of protein crystals deposited on a nanodroplet

S. Nitahara, M. Maeki, H. Yamaguchi, K. Yamashita, M. Miyazaki and H. Maeda, Analyst, 2012, 137, 5730 DOI: 10.1039/C2AN35942A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements