Issue 18, 2012
From the journal:

Analyst

A sandwich assay for quantitative detection of transcription factors in cell lysate

Zhiyuan Fang,a   Wenjuan Zhang,ab   Chenchen Ge,ab   Jie Liu,a   Puchang Liea  and  Lingwen Zeng*a  

* Corresponding authors

a Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
E-mail: zeng6@yahoo.com
Fax: +86 20 32015245
Tel: +86 20 32015245

b University of Science and Technology of China, Hefei, China

Abstract

A double-stranded DNA (dsDNA) mediated sandwich assay was developed for quantitative detection of transcription factors. The detection limit for human recombinant c-jun protein is 2.5 ng, and for c-jun protein the limit is as low as 0.625 μg of cell lysate.

Graphical abstract: A sandwich assay for quantitative detection of transcription factors in cell lysate

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/C2AN35859J
Article type
Communication
Submitted
26 Apr 2012
Accepted
03 Jul 2012
First published
03 Jul 2012

Analyst, 2012,137, 4127-4130

Permissions

Request permissions

A sandwich assay for quantitative detection of transcription factors in cell lysate

Z. Fang, W. Zhang, C. Ge, J. Liu, P. Lie and L. Zeng, Analyst, 2012, 137, 4127 DOI: 10.1039/C2AN35859J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements