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Issue 20, 2012
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Development of a split SNAP-tag protein complementation assay for visualization of proteinprotein interactions in living cells

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Abstract

A split SNAP-tag protein complementation assay was developed for visualization of proteinprotein interactions in living cells. Split SNAP-tagμs, fragments of divided SNAP-tag between amino acid residues 91 and 92, were fused to proteins that can interact with each other. After incubation with a fluorescent SNAP-tag substrate, cells that expressed split SNAP-tag fusion proteins generated fluorescent signals when these proteins interacted. Moreover, by combination with the split CLIP-tag (SNAP-tag mutant), simultaneous labeling was achieved. This split SNAP-tag labeling method should be a useful tool for visualization of proteinprotein interaction processes.

Graphical abstract: Development of a split SNAP-tag protein complementation assay for visualization of protein–protein interactions in living cells

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Publication details

The article was received on 07 Jun 2012, accepted on 03 Aug 2012 and first published on 06 Aug 2012


Article type: Paper
DOI: 10.1039/C2AN35762C
Citation: Analyst, 2012,137, 4760-4765
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    Development of a split SNAP-tag protein complementation assay for visualization of proteinprotein interactions in living cells

    M. Mie, T. Naoki, K. Uchida and E. Kobatake, Analyst, 2012, 137, 4760
    DOI: 10.1039/C2AN35762C

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