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Issue 18, 2012
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Label-free molecular analysis of live Neospora caninum tachyzoites in host cells by selective scanning Raman micro-spectroscopy

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Abstract

A selective scanning method was used to measure spatially resolved Raman spectra of live Neospora caninum tachyzoites colonizing human brain microvascular-endothelial cells. The technique allowed the detection of nucleic acids, lipids and proteins linked to the parasites and their cellular micro-environment at ∼10× shorter acquisition time compared to raster scanning.

Graphical abstract: Label-free molecular analysis of live Neospora caninum tachyzoites in host cells by selective scanning Raman micro-spectroscopy

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Publication details

The article was received on 16 May 2012, accepted on 19 Jul 2012 and first published on 20 Jul 2012


Article type: Communication
DOI: 10.1039/C2AN35640F
Citation: Analyst, 2012,137, 4119-4122
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    Label-free molecular analysis of live Neospora caninum tachyzoites in host cells by selective scanning Raman micro-spectroscopy

    K. Kong, C. J. Rowlands, H. Elsheikha and I. Notingher, Analyst, 2012, 137, 4119
    DOI: 10.1039/C2AN35640F

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