Issue 17, 2012
From the journal:

Analyst

Homogeneous assay for evaluation of aptamer–protein interaction†

Gergely Lautner,a   Zsófia Balogh,c   Anna Gyurkovics,c   Róbert E. Gyurcsányiab  and  Tamás Mészáros*bc  

* Corresponding authors

a Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, H-1111 Budapest, Hungary
E-mail: robertgy@mail.bme.hu
Fax: +36 1 4633408
Tel: +36 1 4631592

b Research Group for Technical Analytical Chemistry of the Hungarian Academy of Sciences, Szt. Gellért tér 4, H-1111 Budapest, Hungary

c Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Tűzoltó u. 37-47, H-1094 Budapest, Hungary
E-mail: Tamas.Meszaros@eok.sote.hu

Abstract

We introduce Amplified Luminescent Proximity Homogenous Assay (ALPHA) to assess the KD value of aptamer–protein complexes as demonstrated through the study of apple stem pitting virus coat protein-specific aptamers. This method can be used as a simple, cost-effective method for screening aptamer–target protein interactions during aptamer selection.

Graphical abstract: Homogeneous assay for evaluation of aptamer–protein interaction

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Article information

DOI
https://doi.org/10.1039/C2AN35419E
Article type
Communication
Submitted
28 Mar 2012
Accepted
04 Jul 2012
First published
06 Jul 2012

Analyst, 2012,137, 3929-3931

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Homogeneous assay for evaluation of aptamer–protein interaction

G. Lautner, Z. Balogh, A. Gyurkovics, R. E. Gyurcsányi and T. Mészáros, Analyst, 2012, 137, 3929 DOI: 10.1039/C2AN35419E

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