Jump to main content
Jump to site search

Issue 16, 2012
Previous Article Next Article

A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells

Author affiliations

Abstract

We present the design, synthesis, spectroscopy, and biological applications of PyBor, a new type of fluorescent probe for peroxynitrite detection in aqueous solution and living cells. The probe employs pyrene as the fluorophore, and is equipped with a chemically responsive unit boronate. The fluorescent probe can selectively detect peroxynitrite with fluorimetric determination and high-performance liquid chromatography analyses in aqueous solution and RAW264.7 cells intracellular free extracts. We also study our probe to time dependent peroxynitrite release from 3-morpholinylsydnonimine hydrochloride. Confocal microscopy experiments using mouse macrophage cell line RAW264.7 show that PyBor is able to detect the different intracellular peroxynitrite levels. In addition, we have performed quantum chemical calculations with TD-DFT/M06/TZVP level with COSMO solvation model basis sets using a suite of Gaussian 09 programs to provide insights into the structure optical properties of PyBor and PyOH.

Graphical abstract: A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Feb 2012, accepted on 25 May 2012 and first published on 30 May 2012


Article type: Paper
DOI: 10.1039/C2AN35246J
Citation: Analyst, 2012,137, 3740-3749
  •   Request permissions

    A fluorescent probe directly detect peroxynitrite based on boronate oxidation and its applications for fluorescence imaging in living cells

    F. Yu, P. Song, P. Li, B. Wang and K. Han, Analyst, 2012, 137, 3740
    DOI: 10.1039/C2AN35246J

Search articles by author

Spotlight

Advertisements