Carbonate interlayered hydrotalcites-enhanced peroxynitrous acid chemiluminescence for high selectivity sensing of ascorbic acid

Abstract

In this study, Mg-Al-carbonate layered double hydroxides (denoted as Mg-Al-CO₃ LDHs) were found to catalyze the chemiluminescence (CL) emission from peroxynitrous acid (ONOOH). The enhanced CL signals resulted from the concentration of peroxynitrite (ONOO⁻) onto the LDHs surface by electrostatic attraction, meaning that ONOO⁻ can interact with the intercalated carbonate easily and effectively. Moreover, ascorbic acid can react with ONOO⁻, or its decomposition products (e.g., ˙OH and ˙NO₂), resulting in a decrease in the CL intensity from the Mg-Al-CO₃ LDHs-catalyzed ONOOH reaction. Based on these findings, a sensitive, selective and rapid CL method was developed for the determination of ascorbic acid using Mg-Al-CO₃ LDHs-catalyzed ONOOH as a novel CL system. The CL intensity was proportional to the concentration of ascorbic acid in the range from 5.0 to 5000 nM. The detection limit (S/N = 3) was 0.5 nM and the relative standard deviation (RSD) for nine repeated measurements of 0.1 μM ascorbic acid was 2.6%. This method has been successfully applied to determine ascorbic acid in commercial liquid fruit juices with recoveries of 97–107%. This work is not only of importance for a better understanding of the unique properties of LDHs-catalyzed CL but also of great potential for extensive applications in many fields, such as luminescence devices, bioanalysis, and labeling probes.