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Issue 16, 2012
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Conformational analysis of single polymer chains in three dimensions by super-resolution fluorescence microscopy

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Abstract

A single polymer chain was directly observed via a super-resolution optical imaging technique, photo-activated localization microscopy (PALM). A hydrophobic rhodamine spiroamide derivative enabled the PALM observation of synthetic polymers. The conformation of a single chain of poly(butyl methacrylate) was investigated with a spatial resolution of 15 nm. The distribution functions of the chain conformation were directly obtained from the high-resolution image of individual chains. Moreover, the three-dimensional structure of individual chains was successfully observed with a lateral spatial resolution of 20 nm and a depth resolution of 40 nm.

Graphical abstract: Conformational analysis of single polymer chains in three dimensions by super-resolution fluorescence microscopy

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Supplementary files

Article information


Submitted
21 Nov 2011
Accepted
07 Feb 2012
First published
02 Mar 2012

Soft Matter, 2012,8, 4390-4395
Article type
Paper

Conformational analysis of single polymer chains in three dimensions by super-resolution fluorescence microscopy

H. Aoki, K. Mori and S. Ito, Soft Matter, 2012, 8, 4390
DOI: 10.1039/C2SM07227K

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