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Issue 17, 2012
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X-Ray propagation imaging of a lipid bilayer in solution

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Abstract

We have used X-ray propagation imaging to visualize a less than 5 nm thick native lipid bilayer membrane freely suspended in aqueous solution. Contrast is formed by free space propagation of hard X-rays, with the membrane illuminated by a nano-focused, partially coherent synchrotron beam, at a controllable distance (defocus) behind the focal spot. Quantitative fitting of the magnified Fresnel fringes shows the transition from membranes swollen with solvent to the native bilayer, containing structural information at near-molecular resolution along the dimension perpendicular to the bilayer. We show first applications of this hybrid technique of propagation imaging and near-field diffraction to the investigation of ultra-thin organic films formed in micro-fluidic devices, namely the formation of a lipid bilayer by the adhesion of two constitutive monolayers.

Graphical abstract: X-Ray propagation imaging of a lipid bilayer in solution

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Supplementary files

Article information


Submitted
05 Jan 2012
Accepted
14 Feb 2012
First published
13 Mar 2012

This article is Open Access

Soft Matter, 2012,8, 4595-4601
Article type
Paper

X-Ray propagation imaging of a lipid bilayer in solution

A. Beerlink, S. Thutupalli, M. Mell, M. Bartels, P. Cloetens, S. Herminghaus and T. Salditt, Soft Matter, 2012, 8, 4595
DOI: 10.1039/C2SM00032F

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